Part:BBa_K5115064

hox and hyp, with EP targeted hoxU

Introduction

Compared with hox and hyp, with EP targeted hoxF, there is only one difference: one links EP with hoxF and another links EP with hoxU. EP ligation of different subunits leads to different effects of hydrogenase's entry into the carboxysome, which further affects the effect of our overall design. After comparison between experiments of these two designs, we make sure that the hoxF-linking-EP one is more satisfying. So, to learn more about the finally adopted part, please click on hox and hyp, with EP targeted hoxF.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 654
  Illegal BglII site found at 1472
  Illegal BglII site found at 1765
  Illegal BglII site found at 10143
  Illegal BglII site found at 10221
  Illegal BamHI site found at 2059
  Illegal XhoI site found at 99
  Illegal XhoI site found at 2266
  Illegal XhoI site found at 4505
  Illegal XhoI site found at 6334
  Illegal XhoI site found at 7496
  Illegal XhoI site found at 9335
  Illegal XhoI site found at 10151
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 533
  Illegal NgoMIV site found at 1071
  Illegal NgoMIV site found at 1281
  Illegal NgoMIV site found at 1593
  Illegal NgoMIV site found at 2359
  Illegal NgoMIV site found at 3039
  Illegal NgoMIV site found at 5646
  Illegal AgeI site found at 6821
  Illegal AgeI site found at 10104
  Illegal AgeI site found at 11103
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4159
  Illegal BsaI site found at 9925
  Illegal BsaI site found at 10087
  Illegal BsaI.rc site found at 702
  Illegal BsaI.rc site found at 1206
  Illegal SapI.rc site found at 10036