Composite
Part:BBa_K5115064
Designed by: Yi Shi Group: iGEM24_Fudan (2024-09-19)
hox and hyp, with EP targeted hoxU
Introduction
This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system:pRAP. It's worth mention that, in this part, the subunit hoxU is fused with EP, check BBa_K5115059(hoxU fused with EP)for more details. To learn more about the hydrogenase, please check BBa_K5115020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .
Usage and biology
This part is crucial in BBa_K5115066(mineral, U module), click on the link to view the experiment details.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 654
Illegal BglII site found at 1472
Illegal BglII site found at 1765
Illegal BglII site found at 10143
Illegal BglII site found at 10221
Illegal BamHI site found at 2059
Illegal XhoI site found at 99
Illegal XhoI site found at 2266
Illegal XhoI site found at 4505
Illegal XhoI site found at 6334
Illegal XhoI site found at 7496
Illegal XhoI site found at 9335
Illegal XhoI site found at 10151 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 533
Illegal NgoMIV site found at 1071
Illegal NgoMIV site found at 1281
Illegal NgoMIV site found at 1593
Illegal NgoMIV site found at 2359
Illegal NgoMIV site found at 3039
Illegal NgoMIV site found at 5646
Illegal AgeI site found at 6821
Illegal AgeI site found at 10104
Illegal AgeI site found at 11103 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4159
Illegal BsaI site found at 9925
Illegal BsaI site found at 10087
Illegal BsaI.rc site found at 702
Illegal BsaI.rc site found at 1206
Illegal SapI.rc site found at 10036
References
[edit]
Categories
Parameters
None |