Regulatory

Part:BBa_K5119017

Designed by: Dhruv Aggarwal   Group: iGEM24_Austin-utexas   (2024-09-27)
Revision as of 03:07, 29 September 2024 by Dhruv (Talk | contribs)


SpcR FL Promoter



This part is a full-length spectinomycin antibiotic resistance promoter originally found in Streptomyces spectabilis and obtained from pBTK403, a broad-host-range bacterial origin plasmid (Leonard et al., 2018). Through our research, we offer a collection of parts (BBa_K5119000 to BBa_K5119087) that enables researchers to assemble their own plasmid that can replicate in both gram-positive species and E. coli, with the added functionality of secreting enzymes capable of degrading gliadin. Explore the entire collection of parts associated with our project on the Parts page of the UT Austin 2024 iGEM Wiki.



Introduction

About 1% of the world population is affected by celiac disease (Lebwohl et al., 2018), an autoimmune disorder triggered by the ingestion of gluten, a protein commonly found in wheat, barley, and rye (Celiac Disease Foundation, 2024). This immune response can cause significant intestinal damage from chronic inflammation, nutrient malabsorption, and even lactose intolerance, making it crucial to find effective treatments. This is further underscored by the widespread presence of gluten in the human diet. The UT Austin 2024 iGEM team seeks to alleviate the burden of celiac disease by developing a collection of parts capable of secreting proteases in a bacterium specifically designed to degrade gliadin, the primary immunogenic component of gluten (Barone et al., 2014). By engineering this bacterium to break down gliadin in a sustained and localized manner, the team aims to prevent the harmful effects of accidental gluten ingestion, offering a solution to improve the lives of individuals with celiac disease. For more details, please visit our project description page.
Figure 1: The UT-Austin 2024 iGEM parts collection. This collection includes 22 constitutive antibiotic resistance promoters (Type 2), 9 secretion tags (Type 3a), 2 reporter proteins and 4 reporter proteins & enzymes (Type 3b), a rpoC terminator (Type 4), and 3 plasmid backbones (Type 56781). Created with Biorender.com."

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None