Translational_Unit

Part:BBa_K5490032

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 00:31, 29 September 2024 by Tzonissss13 (Talk | contribs)


Responsive to synthetic NFAT CasRx

There is 1) a type of promoter with high specificity to a particular type of transcription factor, in this case, the synthetic NFAT, it achieves this by having various upstream elements of a small DNA sequence that contains the TATA box where polymerase II will bind 2) downstream, it contains a CasRx gene , a HA tag, an IRES2 RBS and a mCherry fused with NLS .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1335
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 4754
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1335
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 4754
    Illegal NotI site found at 141
    Illegal NotI site found at 211
    Illegal NotI site found at 506
    Illegal NotI site found at 576
    Illegal NotI site found at 3744
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1335
    Illegal EcoRI site found at 1755
    Illegal BamHI site found at 3735
    Illegal BamHI site found at 3809
    Illegal XhoI site found at 806
    Illegal XhoI site found at 3210
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1335
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 4754
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1335
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 4754
    Illegal NgoMIV site found at 1023
    Illegal AgeI site found at 7
    Illegal AgeI site found at 347
    Illegal AgeI site found at 372
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None