Plasmid_Backbone

Part:BBa_K5109060:Design

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-28)
Revision as of 20:21, 28 September 2024 by Lisafaccincani (Talk | contribs)

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We designed primers forward and reverse overlapping the restriction site, both of them with a single mismatch on the second T of the site "CATATG".

Primer forward: 5’ – CCGCATAAGGTGCACTCTCAGTACAAT – 3’

Primer reverse: 5’ – GTGCACCTTATGCGGTGTGAAATACCG – 3’

After the mutagenesis, the restriction site was changed from CATATG to CATAAG.