Composite

Part:BBa_K5107006

Designed by: Georgios Retsinias   Group: iGEM24_DTU-Denmark   (2024-09-28)
Revision as of 21:56, 28 September 2024 by Georgiosr (Talk | contribs)


T7-HRE5-dB-T

HRE5-3WJdB is recognised by the steroid androgen, progenesterone and glycocorticoid hormone receptor.

Usage and Biology

HRE5 is used in the in cell assay as binding site of the hormones receptors. This recruitment trigger the Lac-Z expression, reporting the presence of the hormone or edc in the cell environment.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 60
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[edit]
Categories
Parameters
None