RBS

Part:BBa_K5097012

Designed by: Christopher Dorce   Group: iGEM24_Oneonta   (2024-09-25)
Revision as of 12:00, 30 September 2024 by Dorcc343 (Talk | contribs)

RBS B0034 with added spacer sequencers

RBS BBa_B0034 with spacer nucleotides

Usage and Biology

This part is a modified form of the Anderson Collections RBS B0034. Additional nucleotides have been added to the 5’ and 3’ ends of the RBS, the sequence of which is AAGAGGAGA. The purpose of these additional nucleotides is to mimic the spacing created by the RCF10 assembly scars.

Figure 1: Alignment of RBS BBa_B0034 (top) with BBa_B0034 (bottom). Alignment generated using link=https://www.genome.jp/tools-bin/clustalw.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None