Part:BBa_K5115061

ribozyme+RBS+hoxF-GS-EP+stem-loop

Introduction

This composite part is composed of hoxF-GS-EP and improved ribozyme-assisted polycistronic co-expression system: pRAP. Get details about hoxF in BBa_K5115020(hox and hyp operon) and EP in BBa_K5115002(EP).

Usage and Biology

Binding hoxF and EP can tether hydrogenase genes to the α-carboxysome, providing a protective microenvironment for the hydrogenase enzyme, enhancing its catalytic activity and stability. The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 654
  Illegal BglII site found at 1472
  Illegal BglII site found at 1765
  Illegal XhoI site found at 99
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 533
  Illegal NgoMIV site found at 1071
  Illegal NgoMIV site found at 1281
  Illegal NgoMIV site found at 1593
  Illegal AgeI site found at 2548
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 702
  Illegal BsaI.rc site found at 1206