Coding

Part:BBa_K5335006:Design

Designed by: Haozhen Liu   Group: iGEM24_HZAU-China   (2024-09-28)
Revision as of 08:41, 28 September 2024 by Adown (Talk | contribs) (→‎References)

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Cry6Aa2 is a Nematocidal crystal protein from Bacillus thuringiensis YBT-1518


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1087
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 982
    Illegal AgeI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural Cry6Aa2 insecticidal crystal protein is expressed in Bacillus thuringiensis, it is best to carry out corresponding codon optimization when expressed in Escherichia coli or saccharomyces cerevisiae.

Source

Cry6Aa2 comes from the genome of Bacillus thuringiensis YBT-1518.

References

Huang J, Guan Z, Wan L, Zou T, Sun M. Crystal structure of Cry6Aa: A novel nematicidal ClyA-type α-pore-forming toxin from Bacillus thuringiensis. Biochem Biophys Res Commun. 2016 Sep 9;478(1):307-313. doi: 10.1016/j.bbrc.2016.07.002. Epub 2016 Jul 2.