Part:BBa_K5398611

LicT^CAT-ViViD fusion protein

LicT^CAT-ViViD fusion protein, also called LicV, is a transcriptional antitermination protein. It is composed of an N-terminal RNA-binding domain (coantiterminator (CAT)) derived from LicT and Vivid(VVD), a small LOV domain-containing protein that could form a dimeric structure quickly in blue light. Illumination could facilitate the dimerization of the fusion protein, consequently augmenting its affinity for the ribonucleic antiterminator (RAT) RNA sequence, thus preventing the formation of an RNA terminator stem–loop structure. When the light is removed, LicV doesn't bind to the RAT sequence, allowing the formation of the terminator hairpin structure which prevents the expression of genes downstream.

Fig. 2 | Improved EL222 system with LicV and RAT.

Usage and Biology

The EL222-based blue light-inducible system allows precise control of gene expression by dimerizing and binding DNA upon blue light exposure. To reduce system leakage, a LicV fusion protein and RAT sequence were introduced, enhancing control by preventing premature transcription termination. This system is ideal for applications requiring tight regulation of gene expression in response to light, such as biosensors and metabolic engineering.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]