Primer

Part:BBa_K5079016

Designed by: Marianna Florez   Group: iGEM24_Queens-Canada   (2024-09-28)
Revision as of 09:16, 30 September 2024 by Jasonsong (Talk | contribs)


K45_CP_N114 Switch primers into pET-28a Switch 8 (Rev)

This is the reverse primer for Part:BBa_K5079033. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: GGTGCTCGAGTGCGGCCGCAGA TGAcaactggccacagtc. 3’ GC Content is 56%, 3' length is 18nt and 3' Tm is 65°C.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None