Part:BBa_K5184053
HrpN ori
In order to rejuvenate cultivations infected by T.urticae, HrpN ori is incorporated in our project with reparation and improvement means. HrpN ori is a refinement of its precursor HrpN, with enhanced solubility in expression. HrpN is thermally stable and produced by gram-negative plant pathogenic bacteria. Once applied exogenously to cultivations, several beneficial responses could be induced including systemic acquired resistance, hypersensitive response effect (HR) and plant growth via stimulating an increase in root and shoot biomasses. Likewise, harpin proteins are competent to enhance plant disease resistance via mutual coordination and plant defense response against diverse pathogens and insects. As an omnipotent treatment for plants in all conditions, HrpN ori provides future iGEM teams dedicate in incorporations of plant health intervention strategies an appealing and effective choice.
Usage and Biology
HrpN ori is a recombinant form of harpin protein, with truncated harpin peptides only containing the N-terminal 103 amino acids or the C-terminal 214 amino acids of harpin, also retained their bioactivity. In this way, harpin protein could be expressed in soluble form. Harpins, first found in Erwinia amylovora, contain several α-helical regions and have an acidic isoelectric point (pI). Once applied exogenously, it is capable of triggering the hypersensitive response in plants, characterized by the rapid death of plant cells at the site of pathogen invasion. This response is a defense mechanism to limit pathogen spread,. Harpins activate various plant defense pathways, including the production of reactive oxygen species (ROS) and the expression of defense-related genes. Beyond local responses, harpins can enhance systemic acquired resistance, a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms, thus leading to an enduring resistant to a broad range of pathogens.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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