Composite

Part:BBa_K5311016

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)
Revision as of 10:13, 27 September 2024 by Carlospdag (Talk | contribs)

MG_010 + RNA thermometer ForU + sfGFP + His-tag

Genetic construct created to produce sfGFP to induce fluorescence in C. acnes and E. coli. This expression is regulated with an RNA Thermometer that allows expression when the optimal temperature of 37°C is achieved

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1686
    Illegal BglII site found at 2916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 3407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1517
    Illegal BsaI.rc site found at 3620


[edit]
Categories
Parameters
None