Composite
Part:BBa_K5299202:Design
Designed by: Maria - Asimina Kakadiari Group: iGEM24_Thessaly (2024-09-23)
BBa_J23119 - BBa_B0030 - BBa_I746916 - BBa_B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 64
Design Notes
Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.
Source
The promoter BBa_J23119 already existed in the Registry. The RBS BBa_B0030 already existed in the Registry. The sfGFP BBa_I746916 already existed in the Registry. The BBa_B0015 already existed in the Registry.
References
- [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491
- [2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology, 24 (1), 79–88. doi:10.1038/nbt1172