Coding

Part:BBa_K5276002:Design

Designed by: Zitao Qiu   Group: iGEM24_UCAS-China   (2024-09-26)
Revision as of 03:45, 28 September 2024 by Qiuzitao (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Bxb1 recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300


Design Notes

Source

References

Roquet, N., Soleimany, A. P., Ferris, A. C., Aaronson, S., & Lu, T. K. (2016). Synthetic recombinase-based state machines in living cells. Science, 353(6297), aad8559. doi:10.1126/science.aad8559

Grindley N. D. (1997). Site-specific recombination: synapsis and strand exchange revealed. Current biology : CB, 7(10), R608–R612. https://doi.org/10.1016/s0960-9822(06)00314-9