Part:BBa_K5246052

H.Baltica hfsA-hfsB-hfsD-hfsF-hfsC-hfsI Polysaccharide export apparatus

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 6069
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7303
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2647
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 6069
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 6069
  Illegal NgoMIV site found at 2186
  Illegal AgeI site found at 5527
  Illegal AgeI site found at 6868
  
• 1000
  COMPATIBLE WITH RFC[1000]