Coding

Part:BBa_K5291026:Design

Designed by: Haosheng Lin   Group: iGEM24_BNUZH-China   (2024-09-25)
Revision as of 15:50, 28 September 2024 by Kendy (Talk | contribs) (→‎References)


pntB: β subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Considerations in the design of pntB include their important role in catalytic hydrogen transfer between NADH and NADPH.


Source

Escherichia coli str. K-12 substr. MG1655

References

1.Clarke DM, Loo TW, Gillam S, Bragg PD. Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli. Eur J Biochem. 1986 Aug 1;158(3):647-53. doi: 10.1111/j.1432-1033.1986.tb09802.x. PMID: 3525165.
2.Shi A, Zhu X, Lu J, Zhang X, Ma Y. Activating transhydrogenase and NAD kinase in combination for improving isobutanol production. Metab Eng. 2013 Mar;16:1-10. doi: 10.1016/j.ymben.2012.11.008. Epub 2012 Dec 14. PMID: 23246519.