Plasmid_Backbone

Part:BBa_K5293015

Designed by: Arnaud Boudigou   Group: iGEM24_uOttawa   (2024-09-23)
Revision as of 13:22, 30 September 2024 by Arnaud-boudigou (Talk | contribs)


pHREAC_eGFP_VAC

pHREAC plasmid backbone for Vacuole targeted protein expression. Sap I sites are used to clone you insert (GGC & AAC are the scars) AAC Scar is part of the CTPP localisation tag. This empty plasmid backbone contains an insert not displayed on the sequence for easier cloning with mCherry (BBa_K5293020) as a screening tool

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2344
    Illegal EcoRI site found at 4042
    Illegal XbaI site found at 940
    Illegal SpeI site found at 5888
    Illegal PstI site found at 8545
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2344
    Illegal EcoRI site found at 4042
    Illegal NheI site found at 3253
    Illegal SpeI site found at 5888
    Illegal PstI site found at 8545
    Illegal NotI site found at 5742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2344
    Illegal EcoRI site found at 4042
    Illegal BglII site found at 1307
    Illegal BglII site found at 2570
    Illegal BglII site found at 2590
    Illegal BglII site found at 9360
    Illegal BamHI site found at 946
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2344
    Illegal EcoRI site found at 4042
    Illegal XbaI site found at 940
    Illegal SpeI site found at 5888
    Illegal PstI site found at 8545
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2344
    Illegal EcoRI site found at 4042
    Illegal XbaI site found at 940
    Illegal SpeI site found at 5888
    Illegal PstI site found at 8545
    Illegal NgoMIV site found at 3054
    Illegal NgoMIV site found at 4212
    Illegal NgoMIV site found at 4738
    Illegal NgoMIV site found at 5614
    Illegal NgoMIV site found at 5738
    Illegal NgoMIV site found at 6765
    Illegal NgoMIV site found at 7356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None