Part:BBa_K191004

TRP promoter - RBS - RFP - Term

Part BBa_K191004 consists of the Trp promoter followed by the RFP gene. In E. coli, the Trp promoter is situated in front of the Trp operon, which contains the genes necessary for the tryptophan biosynthetic pathway. In brief, genes placed after the Trp promoter should be repressed in the presence of tryptophan.

Usage and Biology

This part permits characterization of part BBa_K191003 as it contains Trp promoter sequence, to which the fused protein LovTAP can bind. For complete genetic circuit, see here.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  Illegal AgeI site found at 666
  Illegal AgeI site found at 778
  
• 1000
  COMPATIBLE WITH RFC[1000]