Plasmid_Backbone
Part:BBa_K5071022
Designed by: SIHAN JING Group: iGEM24_SubCat-China (2024-09-23)
pRSFDuet-1 backbone
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NotI site found at 149 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BglII site found at 305
Illegal BamHI site found at 106
Illegal XhoI site found at 354 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NgoMIV site found at 324
Illegal AgeI site found at 566 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
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BBa_K5071022 (pRSFDuet-1 backbone)
Name
pRSFDuet-1 backbone
Base Pairs
3587 bp
Origin
Escherichia coli
Usage and Biology
The pRSFDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pRSFD, and we used this vector for the connection of the target genes.
Cultivation
The pRSFDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pRSFD, with a length of 3587 bp. Fig 1. shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.
Fig 1. The purpose segment of backbone pRSFDuet-1
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Categories
Parameters
| None |