Part:BBa_K5301015

NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.

NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.spNW50 could construct nanodiscs with large diameter, and is circularized by SpyTag-Spycatcher.

Figure 1. Electron microscopic images of nanodisks with a particle size of about 100nm prepared by NW50. The particle size is in agreement with our DLS results.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 496
  Illegal EcoRI site found at 1576
  Illegal SpeI site found at 448
  Illegal PstI site found at 664
  Illegal PstI site found at 1204
  Illegal PstI site found at 1237
  Illegal PstI site found at 1744
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 496
  Illegal EcoRI site found at 1576
  Illegal NheI site found at 64
  Illegal SpeI site found at 448
  Illegal PstI site found at 664
  Illegal PstI site found at 1204
  Illegal PstI site found at 1237
  Illegal PstI site found at 1744
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 496
  Illegal EcoRI site found at 1576
  Illegal BglII site found at 924
  Illegal BglII site found at 1464
  Illegal BglII site found at 1632
  Illegal BamHI site found at 97
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 496
  Illegal EcoRI site found at 1576
  Illegal SpeI site found at 448
  Illegal PstI site found at 664
  Illegal PstI site found at 1204
  Illegal PstI site found at 1237
  Illegal PstI site found at 1744
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 496
  Illegal EcoRI site found at 1576
  Illegal SpeI site found at 448
  Illegal PstI site found at 664
  Illegal PstI site found at 1204
  Illegal PstI site found at 1237
  Illegal PstI site found at 1744
  
• 1000
  COMPATIBLE WITH RFC[1000]