Protein_Domain
tolRII

Part:BBa_K257005:Design

Designed by: Guillaume Cambray   Group: iGEM09_Paris   (2009-08-20)
Revision as of 15:58, 21 October 2009 by Sboumahdi (Talk | contribs) (→‎Design Notes)

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TolR domain II N-term fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Product created by PCR amplification. For fusion of proteins to the N-terminal extremity of TolRII using the silver fusion standard RFC23. The suffix used to create this parts conform to the std 10 guidelines for coding DNA. This part is inserted in the topo vector.

Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References