Coding

Part:BBa_K5246029:Design

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-22)
Revision as of 18:08, 28 September 2024 by Sarpilo (Talk | contribs) (→‎Design Notes)

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CB2/CB2A HfsL Glycosyltransferase, 6xHis tag for purification


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Plasmids were cloned using Golden Gate assembly, inserting the HfsL gene into the pRSFDuet backbone.

Source

Caulobacter crescentus (Caulobacter vibroides) CB2 (DSM 4727, ATCC 15252) Caulobacter crescentus (Caulobacter vibroides) CB2A (DSM 25117)

References