Part:BBa_K5246028
CB2/CB2A HfsK Acetyltransferase, 6xHis tag for purification
Introduction
Usage and Biology
TBA
This part also has a non his-tagged variant BBa_K5246011.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 94
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 94
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 823
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 94
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 94
- 1000COMPATIBLE WITH RFC[1000]
Experimental characterization
Bioinformatic analysis
Using CDD analysis, it was identified that HfsK is similar to the GNAT N-acetyltransferase family. Its domains suggest that HfsK is part of the Bcls superfamily. Acetyltransferases of this superfamily are usually involved in cellulose biosynthesis. Protein BLAST did not give conclusive results, which could result from a unique HfsK protein amino acid sequence and structure.
DeepTMHMM's protein topology predictions showed that HfsK is most likely a globular protein located on the cytoplasmic side of the membrane.
High confidence scores of AlphaFold 3 structures suggest that HfsK is likely a globular protein. A pTM score above 0.5 suggests that the predicted overall structure may closely resemble the true protein fold, while ipTM indicates the accuracy of the subunit positioning within the complex. Values higher than 0.8 represent confident, high-quality predictions (Fig.1).
To summarise, HfsK is most likely a globular N-acetyltransferase. Earlier evidence, combined with our findings, suggests that it plays a role in the deacetylation of N-acetylglucosamine within the holdfast synthesis pathway. [1][2][3]
References
1. Chepkwony, N.K. and Brun, Y.V. (2021) ‘A polysaccharide deacetylase enhances bacterial adhesion in high-ionic-strength environments’, iScience, 24(9), p. 103071. doi:10.1016/j.isci.2021.103071.
2. Sprecher, K.S. et al. (2017) ‘Cohesive properties of the Caulobacter crescentus holdfast adhesin are regulated by a novel C-di-GMP effector protein’, mBio, 8(2). doi:10.1128/mbio.00294-17.
3. Hershey, D.M., Fiebig, A. and Crosson, S. (2019) ‘A genome-wide analysis of adhesion in Caulobacter crescentus identifies new regulatory and biosynthetic components for holdfast assembly’, mBio, 10(1). doi:10.1128/mbio.02273-18.
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