Coding

Part:BBa_K5246010

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-22)
Revision as of 13:39, 22 September 2024 by Zaboras26 (Talk | contribs)


CB2/CB2A HfsJ Glycosyltransferase

Introduction

Usage and Biology

Gene HfsJ from Caulobacter crescentus encodes a protein of 316aa. HfsJ is one of the glycosiltransferases involved in holfast synthesis pathway and is structurally very similar to glycosiltransferases that transfer UDP-N-acetyl-D-mannosaminuronic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 632
    Illegal BamHI site found at 780
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 353
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Experimental characterization

References

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Categories
Parameters
None