Coding

Part:BBa_K5246015

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-22)
Revision as of 09:45, 22 September 2024 by Zaboras26 (Talk | contribs)


HB HfsC Polysaccharide polymerase

Introduction

Usage and Biology

Gene HfsC from Hirschia baltica, encoding a protein of 454 amino acids polymerases repeats of monomers into a mature holdfast polymer. Deletion of polysaccharide polymerase gene hfsC in C.Crescentus didn't cause holdfast synthesis defects, because of its paralogue - HfsI. Double mutants of HfsC and HfsI cause severe holfast synthesis defects

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 889
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Experimental characterization

References

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Categories
Parameters
None