Coding
Part:BBa_K5246015
Designed by: Edgaras Zaboras Group: iGEM24_Vilnius-Lithuania (2024-09-22)
HB HfsC Polysaccharide polymerase
Introduction
Usage and Biology
Gene HfsC from Hirschia baltica, encoding a protein of 454 amino acids polymerases repeats of monomers into a mature holdfast polymer. Deletion of polysaccharide polymerase gene hfsC in C.Crescentus didn't cause holdfast synthesis defects, because of its paralogue - HfsI. Double mutants of HfsC and HfsI cause severe holfast synthesis defects
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 889
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Experimental characterization
References
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Categories
Parameters
None |