Coding

Part:BBa_K5246022

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-22)
Revision as of 08:48, 22 September 2024 by Zaboras26 (Talk | contribs)


HB HfsJ Glycosyltransferase


Introduction

Usage and Biology

Gene HfsJ from Hirschia baltica encodes a protein of 260 amino acids. HfsJ is one of the glycosyltransferases involved in holdfast synthesis pathway and is structurally very similar to glycosyltransferases that transfer UDP-N-acetyl-D-mannosaminuronic acid. Ir belongs to the WecB/TagA/CpsF family of glycosyltransferases that catalyze the formation of glycosidic bonds and may be involved in the biosynthesis of repeating polysaccharide units found in membrane glycolipids. It is predicted that this is an intracellular protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 188
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 188
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 188
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 188
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Experimental characterization

References

[edit]
Categories
Parameters
None