Coding
Part:BBa_K5246020
Designed by: Edgaras Zaboras Group: iGEM24_Vilnius-Lithuania (2024-09-22)
HB HfsH Deacetylase
Introduction
Usage and Biology
The polysaccharide deacetylase HfsH is required for H. baltica adhesion. Holdfast polysaccharides in H. baltica HfsH mutants lack cohesive and adhesive properties. Research instigating Hirschia baltica genome, found that hfsH is in the hfs locus while hfsK and its paralogs are found outside the hfs locus. Colour coding corresponds to homologs and paralogs. Hash marks indicate genes that are found in a different location in the genome. [1] HfsH expression correlates positively with holdfast binding in high ionic strength. HfsH is an important factor for adherence in high ionic-strength environments, adhesion and biofilm formation. It is also crucial for retention of holdfast thiols and galactose monosaccharides. [1]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 385
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Experimental characterization
References
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Categories
Parameters
None |