Translational_Unit

Part:BBa_K216008:Design

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-16)
Revision as of 16:17, 21 October 2009 by Cfrench (Talk | contribs) (→‎Source)

bacterial luciferase LuxAB of Xenorhabdus luminescens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1049


Design Notes

No special considerations. Please note that preliminary sequencing suggests that there may be a problem with the SpeI site of the construct submitted. If this is confirmed, we will submit a revised version and remove this note.


Source

Amplified from genomic DNA of Xenorhabdus luminescens strain Hb, obtained from the National Collection of Industrial and Marine Bacteria, Averdeen, UK, strain NCIMB 12670.

References

  • Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound. Journal of Bacteriology 173, 1399-1405.
  • Valkova, N., Szittner, R., and Meighen, E.A. 1999. Control of luminescence decay and flavin binding by the LuxA carboxy-terminal regions in chimeric bacterial luciferases. Biochemistry 38, 13820-13828.
  • Westerlund-Karlsson, A., Saviranta, P., and Karp, M. 2002. Generation of thermostable monomeric luciferases from Photorhabdus luminescens. Biochemical and Biophysical Research Communications 296, 1072-1076.
  • Mitchell, R.J., Ahn, J.M., and Gu, M.B. 2005. Comparison of Photorhabdus luminescens and Vibrio fischeri lux fusions to study gene expression patterns. Journal of Microbiology and Biotechnology 15, 48-54.