Device

Part:BBa_K283023:Design

Designed by: Liu Chenli   Group: iGEM09_HKU-HKBU   (2009-10-11)
Revision as of 14:46, 21 October 2009 by NelsonChu (Talk | contribs) (→‎Source)

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plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator

The gene expression cheZ is controled by the cI regulated promoter K094002 which is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor. cI binding results in repression of transcription. Our group intended to use this device to realize speed control system.



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal NheI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal BglII site found at 332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This part is composited from BBa_R0062, BBa_B0034, BBa_K094011, BBa_K094013, BBa_K094002, BBa_B0034, BBa_K094100 and BBa_K094013

References