Part:BBa_K5115080

ribozyme+RBS+nikD+stem-loop

Introduction

This composite part is composed of nikD and improved ribozyme-assisted polycistronic co-expression system: pRAP. The nikD is a subunit of the the NikABCDE transporter, an ATP-binding cassette, made up by nikA, nikB, nikC, nikD and nikE. This composite part will finally be used in BBa_K5115082(ribozyme connected nik operon) for further experiment.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 282
  
• 1000
  COMPATIBLE WITH RFC[1000]