Composite

Part:BBa_K5115035

Designed by: Liyue Chen   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 16:44, 26 September 2024 by Chenliyue (Talk | contribs)


ribozyme+RBS+MTA+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of MTA and improved ribozyme-assisted polycistronic co-expression system: pRAP. The MTA is a protein that can bind with nickel ion and reduce its toxity, get details about MTA in BBa_K5115050(MTA, Metallothionein).

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 198
  • 1000
    COMPATIBLE WITH RFC[1000]


References

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Categories
Parameters
None