Protein_Domain
DigA

Part:BBa_K243003:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-12)
Revision as of 14:05, 21 October 2009 by Timo (Talk | contribs) (→‎Design Notes)

Digoxigenin binding protein (DigA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with Biobrick pre-and suffix for fusion proteins according the RFC 25.
Commented GenBank file

Source

Planed and designed by Team Freiburg Bioware09 andsynthesized by purimex.

References

Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic acids--an overview. Cell Mol Biol. 41(7):883-905.

Roche Applied Science (2002) DIG Application Manual for Nonradioactive In Situ Hybridization (3rdedition)

Moter A, Göbel UB (2000) Fluorescence in situ hybridisation (FISH) for direct visualization of microorganism, J Microbiol Meth. 41: 85-112

http://www.thermo.com/eThermo/CMA/PDFs/Various/File_29495.pdf (image)