Part:BBa_K5115019

ribozyme+RBS+hypF+stem-loop

Introduction

This composite part is composed of hypF and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hypF is a subunit of the hydrogenase made up by hox and hyp, get details about hypF in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 499
  Illegal XhoI site found at 2338
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 104
  Illegal NgoMIV site found at 325
  Illegal NgoMIV site found at 349
  Illegal NgoMIV site found at 710
  Illegal NgoMIV site found at 2110
  Illegal NgoMIV site found at 2510
  
• 1000
  COMPATIBLE WITH RFC[1000]