Part:BBa_K5115018

ribozyme+RBS+hypB+stem-loop

Introduction

This composite part is composed of hypB and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hypB is a subunit of the hydrogenase made up by hypB and hyp, get details about hoxF in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 411
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 100
  Illegal NgoMIV site found at 946
  Illegal AgeI site found at 898
  
• 1000
  COMPATIBLE WITH RFC[1000]