Part:BBa_K5184000

SltNPPS

Targeting both the prevention and extermination of 7 urticae, we aim to produce7-epi-zingiberene (7epiZ),a sesquiterpene that is found to have repellent,fecundity-reducing, and fatal effects towards 7. urticae. In order to synthesize7epiZ from glucose in E. coli, we introduce SltNPPS, a neryl diphosphate synthasewhich catalyzes the formation of neryl diphosphate (NPP) from dimethylallyldiphosphate (DMAPP) and isopentenyl diphosphate (IPP), products of the MVApathway introduced in E. col. Through exploring the usage of SltNPPS, we providefuture iGEM teams with an insight into how to biosynthesize susquiterpenes usingE. coli as the chassis.

Usage and Biology

"SltNPPS is a cis-prenyl transferase from Solanum Lycopersium with its N-terminal 45 amino acid truncated that catalyzes the conversion of geranyl diphosphate into neryl diphosphate, which is an essential step in the biosynthesis of monoterpenes in plants. We constructed a novel sesquiterpene synthesis pathway in E. coli. Using glucose as our raw material, we introduce the MVA pathway, which transforms glucose into dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP). Afterwards, SltNPPS, a neryl diphosphate synthase catalyze the production of NPP from IPP and DMAPP. Mvan4662 is then introduced to catalyze the formation of Z,Z-FPP. In the end, ShZIS transforms Z,Z-FPP into 7epiZ. "

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

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