Reporter

Part:BBa_K216009:Experience

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-16)
Revision as of 13:48, 21 October 2009 by Evangelineoh (Talk | contribs) (→‎Applications of BBa_K216009)

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Applications of BBa_K216009

This part was designed to characterize the activity of the PyeaR promoter by Miller assay, as a backup to the GFP-based construct used in the promoter characterization kit.

Results from Miller's Assay
PyeaR-miller.png

User Reviews

Initial Tests: Edinburgh iGEM 2009: To test this part, we grew E. coli JM109 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of sodium nitrate and sodium nitrite. The following day, we performed Miller assays (LacZ activity assays using o-nitrophenyl galactoside, ONPG, as a chromogenic substrate). The results showed strong induction by both nitrate and nitrite, as expected for this promoter (see biology information for part BBa_K216005).

UNIQ1b373786c30ad3b6-partinfo-00000000-QINU UNIQ1b373786c30ad3b6-partinfo-00000001-QINU