Coding

Part:BBa_K5136026

Designed by: Xiaoxiao Zhang   Group: iGEM24_XMU-China   (2024-09-14)
Revision as of 12:44, 17 September 2024 by Ligenn (Talk | contribs)


cellulase EG5C-His tag


Cellulase EG5C
Biology
Cellulase
Efficient hydrolysis of cellulose to glucose depends on the synchronized action of three classes of enzymes, endoglucanase, exoglucanase and β-glucosidase. Processivity is a common mode of action for many exoglucanases and plays a key role in the complete hydrolysis of crystalline cellulose. In contrast, classic endoglucanases randomly cleave β-1,4-glycosidic bonds in the interior of the cellulosic chains and have been considered to be non-processive. Studies have shown that in contrast to other cellulolytic systems that dependent on both endo and exoglucanases, a processive endoglucanase coupled with a β-glucosidase may be sufficient for the degradation of cellulose. Cellulase EG5C is a kind of proteins found from Bacillus subtilis BS-5 (1). It is hypothesized that B. subtilis BS-5 might produce a processive endoglucanase, which might substitute for the apparent deficiency in exoglucanase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 958
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 620


[edit]
Categories
Parameters
None