Part:BBa_K5136026

cellulase EG5C-His tag

Cellulase EG5C
Biology
Cellulase
Efficient hydrolysis of cellulose to glucose depends on the synchronized action of three classes of enzymes, endoglucanase, exoglucanase and β-glucosidase. Processivity is a common mode of action for many exoglucanases and plays a key role in the complete hydrolysis of crystalline cellulose. In contrast, classic endoglucanases randomly cleave β-1,4-glycosidic bonds in the interior of the cellulosic chains and have been considered to be non-processive. Studies have shown that in contrast to other cellulolytic systems that dependent on both endo and exoglucanases, a processive endoglucanase coupled with a β-glucosidase may be sufficient for the degradation of cellulose. Cellulase EG5C is a kind of proteins found from Bacillus subtilis

BS-5 (1). It is hypothesized that 

B. subtilis BS-5 might produce a processive endoglucanase, which might substitute for the apparent deficiency in exoglucanase activity.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 958
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 610
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 620