Part:BBa_K5115001

RcnR_C35L

Introduction

The Ni-Fe hydrogenase we use is an enzyme that can catalyze not only the reduction of hydrogen ions to hydrogen under anaerobic conditions but also the reversed reaction under aerobic conditions. Among this enzyme, hoxF is a hydrogenase subunit responsible for electron transport, which, together with the hoxU subunit, forms the module of NADH dehydrogenase.

Usage and Biology

HoxF subunit is very important in Ni-Fe hydrogenase

The most important group in hoxF is FMN-b, which has the ability of switching electron. Under anaerobic conditions, NADH is oxidized to NAD⁺ on the surface of hoxF subunit. Under aerobic conditions, NAD⁺ is reduced to NADH on the surface of the hoxF subunit. Under anaerobic conditions, the electrons generated in this reaction travel through a series of processes to the hoxH, completing the final reduction of the hydrogen ion.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 577
  Illegal BglII site found at 1395
  Illegal BglII site found at 1688
  Illegal XhoI site found at 22
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 456
  Illegal NgoMIV site found at 994
  Illegal NgoMIV site found at 1204
  Illegal NgoMIV site found at 1516
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 625
  Illegal BsaI.rc site found at 1129