Part:BBa_K5330021
LgBiTEncapsulin2A
This part along with BBa_K5330020 are the two components of a test for Mycobacterium avium subspecies paratuberculosis (MAP.) This part is composed of a type 2A encapsulin (from MAP) with a linker to a LgBiT. The LgBiT is half of a split luciferase. This acts as a reporter system for cage formation of Encapsulins linked to either LgBiT or SmBiT which when they come together in the presence of NanoGlo reagents, they produce light. With both SmBiT-Encap2A and LgBiT-Encap2A present in the test, light output is detected. When a blood sample of a cow infected with Johnes diseases is introduced into this solution, there should be a detected loss of light. This is because Encapsulin monomers from MAP will rearrange to incorporate our engineered monomers (SmBiT-Encap2A and LgBiT-Encap2A) resulting in distance being introduced between halves of the split luciferase and either less or no light produced at all.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 588
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 754
Illegal NgoMIV site found at 760 - 1000COMPATIBLE WITH RFC[1000]
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