Part:BBa_K180017

Rock-paper-scissors - Scissors primary plasmid

Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand.

BBa_K180018 reads along reverse strand, and BBa_K180019 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.

mBanana and LuxI are generated under tac pR regulation, whilst LasR is produced constitutively.

Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function as the 'Scissors' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180020, and pairs K180010 & K180013, K180014 & K180016.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 2754
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 887
  Illegal NheI site found at 910
  Illegal PstI site found at 2754
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 2754
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 2754
  Illegal NgoMIV site found at 534
  Illegal NgoMIV site found at 3000
  Illegal AgeI site found at 337
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2943