Regulatory

Part:BBa_K174004

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-10)
Revision as of 14:57, 21 October 2009 by Goksel (Talk | contribs)

Pspac promoter

This promoter can be induced by IPTG and it is tightly regulated by LacI. The wild type lac operator has been replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI more than 2.5 fold. To repress the promoter LacI needs to be expressed. Addition of IPTG will derepress LacI activity.

BBa_K174004 is an enhancement of Biobrick BBa_K090501 and provides a tighter regulation. BBa_K090501 has the operator sequence from pmutin2 with O1 operator. BBa_K143015 and BBa_I746665 biobricks have also the wild type lac operator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Vagner, V., E. Dervyn, et al. (1998). "A vector for systematic gene inactivation in Bacillus subtilis." Microbiology 144(11): 3097-3104.
  2. Lehming, N. et al. The interaction of the recognition helix of Lac repressor with lac operator. EMBO J. 6, 3145−3153 (1987).
[edit]
Categories
//chassis/prokaryote/bsubtilis
//regulation/negative
regulator
Parameters
None