Part:BBa_K5184022
G1M5-SUMO-tag
G1M5 is the mutated, less hydrophobic version of the signal peptide of the G1 cyclomaltodextrin glucanotransferase (CGtase) of Bacillus sp., which allows the extracellular secretion of the bacterial enzyme. The signal peptide can be divided into three regions: an N-terminus region with several positively charged amino acids, a central hydrophobic region, and a hydrophilic cleavage region. The N-terminus region was found to act as intramolecular chaperones, and to be in cooperation with SecA and SecB to act as factors for protein targeting to the membrane.
Constructs containing the signal peptide G1M5 allows extracellular secretion of the fusion protein. In context of our project this will allow export of spider venom peptide into extraceullar spaceafter translation in cytoplasm, of which can lower production cost and make production process more efficient. This exporting process can also provide a more oxidative environment that aids the formation of disulphide bridges that is essential for the correct folding of spider venom peptides."
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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