Part:BBa_K5398030
A fusion protein that adheres to the surface of dentate ring protein and substrate of squid.
In the Mfp5-TRn4 fusion proteins, Mfp5 is derived from the mussel foot proteins, whose tyrosine residues are oxidized by tyrosinase into dopamine, which primarily forms π-π bonds and hydrogen bonds with the surface materials, allowing them to adhere to various materials. TRn4 is a protein obtained by repeating the sequence from squid ring teeth proteins four times, and the β-sheet on its structure can connect with the β-sheet on the structure of highly repetitive squid ring teeth proteins through hydrogen bonds. Tyrosine on Mfp5 generates dopa when tyrosinase is present, which makes Mfp5-TRn4 fusion protein adhere to the surface materials.
Fig. 1 The plasmid map of TRn4-mfp5.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1035
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 189
Illegal XhoI site found at 1018 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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