Coding

Part:BBa_K5396002

Designed by: Alex Johan Mendes Comodaro   Group: iGEM24_CNPEM-BRAZIL   (2024-09-06)
Revision as of 16:37, 9 September 2024 by Jocomodaro (Talk | contribs)


Nt2RepCt-SpyTag

Spidroins are the primary proteins that compose spider silk, renowned for their exceptional mechanical properties, including strength, elasticity, and biodegradability. These proteins are produced in specialized glands of spiders and it can have various functions, such as web construction, prey capture, and mobility.

NT2RepCT is a recombinant miniature spidroin protein that has been engineered to mimic the properties of natural spider silk proteins. It is composed of several key domains that enable it to self-assemble into hydrogels and fibers under the right conditions [ ].

The Nt2RepCt protein has a SpyTag, a 13-amino-acid peptide that is a main part of the SpyCatcher-SpyTag system, which can enable irreversible protein conjugation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 895
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None