Coding

Part:BBa_K5396001

Designed by: Alex Johan Mendes Comodaro   Group: iGEM24_CNPEM-BRAZIL   (2024-09-06)
Revision as of 17:16, 9 September 2024 by Jocomodaro (Talk | contribs)


Barbie1_RFP_3xMad10

BARBIE1 is a synthetic protein derived from BaCBM2 (BBa_K5396000) through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.

The BARBIE1 protein is fused with the red fluorescent protein (RFP)[ ], which exhibits an excitation maximum at 558 nm and an emission maximum at 583 nm. This fusion enhances the visualization of BARBIE1 by fluorescence-based methods.


Protein Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 88
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None