Coding
Part:BBa_K5280129
Designed by: Hua XU Group: iGEM24_HKUST-GZ (2024-09-07)
LexRO
LexRO is a recombinant protein which will dimerise in darkness and the dimers will bind to DNA, blocking the expression of downstream genes. When exposed to light, more specifically blue light (450~465nm), the dimers will deassiociate and lose the ability to repress gene expression. Therefore, the downstream genes will be expressed.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514
Illegal NotI site found at 587 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |