Part:BBa_K216016:Experience

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Applications of BBa_K216016

This part is designed to produce a luminescent response in the presence of nitrate or nitrite anions. The bacterial luciferase reaction also requires oxygen and a long chain aldehyde. This can be biosynthesised by the luxCDE gene products (we are preparing a BioBrick with these) or can be supplied in the form of 0.05 - 0.1% v/v n-decanal. Luminescence is blue, around 495 nm (see notes on part BBa_K216008 for more details).

User Reviews

Initial tests: Edinburgh iGEM 2009: This part was tested by growing E. coli JM109/pSB1A2-BBa_K216016 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of nitrate and nitrite (0, 5, 10 mM sodium nitrite, and 10, 20, 40 mM sodium nitrate). The following day, 0.05% (ie 0.5 microlitres/ml) n-decanal was added, and the vials were observed in a dark room. The control, with no nitrate or nitrate, was least bright, possibly non-luminescent. The brightest was the vial with 20 mM nitrate, followed by that with 10 mM nitrate. The others, with 40 mM nitrate or 5 or 10 mM nitrite, were of intermediate brightness. Unfortunately, we don't currently have a luminometer to make a quantitive measurement of luminescence, but in qualitative terms, this part seems to work fine.

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