RBS
Part:BBa_K5117000
Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann Group: iGEM24_TU-Dresden (2024-08-31)
BsRBS
This part consists of the ribosome binding site of Bacillus subtilis (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer.
BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team. This part was neither built nor characterized in the laboratory.
Target organism: Bacillus subtilis
Main purpose of use:: Gene expression and protein production using the host Bacillus subtilis
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Molecular microbiology 6(9), 1105-1114.
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Categories
Parameters
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